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KMID : 0948320050050010012
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Konyang Medical Journal
2005 Volume.5 No. 1 p.12 ~ p.16
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Veratridine Stimulates Amylase Secretion of Isolated Rat Pancreatic Lobules via M₁and M₃Muscarinic Receptors
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Lee Mee-Young
Kim Dong-Kwan
Kim Se-Hun
Park Hyung-Seo
Lee Kyung-Moo
Kim Yun-Mi
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Abstract
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It has been well documented that pancreatic exocrine secretion is stimulated by cholinergic neurotransmitter. This study was aimed to classify the muscarinic receptor subtypes involved in the pancreatic enzyme secretion of the isolated pancreatic lobules activated by intrapancreatic neuron using veratridine. To activate the intrapancreatic neuron, four pancreatic lobules were incubated with veratridine, a sodium channel activator, and the mean percentages of total cellular amylase released over a 90-min incubation were subjected to statistical analysis. When pancreatic lobules incubated with tetrodotoxin, a sodium channel blocker, veratridine-induced pancreatic amylase secretion was completely blocked. Atropine, a muscarinic antagonist, also markedly inhibited the veratridinestimulated pancreatic amylase secretion. However, treatment of hexamethonium, a nicotinic ganglionic antagonist, failed to change the veratridine-stimulated pancreatic amylase secretion. Pirenzepine, a M1 receptor antagonist, and 4- DAMP, a M3 receptor antagonist, significantly reduced the veratridine-induced pancreatic amylase secretion. Treatment of methoctramine, a M2 receptor antagonist, failed to change veratridine-induced pancreatic amylase secretion. These results suggest that M1 and M3 receptor subtypes may be involved in the intrapancreatic cholinergic regulation of exocrine pancreas.
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KEYWORD
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Amylase secretion, Muscarinic receptors, Veratridine, Pirenzepine, Methoctramine, 4-DAMP
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